Ohyama Et Al. (2015)
Understanding brain function and development would be facilitated enormously by being able to perform all experiments on the basis of known circuitry. Over 20 laboratories world wide have contributed towards the reconstruction of neurons in the central nervous system of Drosophila larva, led by the Cardona lab at HHMI Janelia. Here, we see a side view of the approximately 7,000 neurons reconstructed so far, either in full or partially, of the approximately 12,000 neurons of this animal. The 0111-8 data set was originally sectioned and imaged by Richard D. Fetter and his two tech assistants, and funded by the HHMI Janelia Fly EM Project Team. There are now many more papers now using the 0111-8 data (see publications below).
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acardona_0111_8 i
Viz
Example cutout
# pip install numpy tifffile cloud-volume import numpy as np import tifffile from cloudvolume import CloudVolume vol = CloudVolume( "s3://open-neurodata/cardona/acardona_0111_8__12_03_2017/image", mip=0, use_https=True ) # load data into numpy array cutout = vol[14336:14848, 23040:23552, 2416:2432] # save cutout as TIFF tifffile.imwrite("data.tiff", data=np.transpose(cutout))
Documentation for cloud-volume located here
acardona_abd1_5 i
Viz
Example cutout
# pip install numpy tifffile cloud-volume import numpy as np import tifffile from cloudvolume import CloudVolume vol = CloudVolume( "s3://open-neurodata/cardona/acardona_abd1_5/image", mip=0, use_https=True ) # load data into numpy array cutout = vol[10752:11264, 9216:9728, 96:112] # save cutout as TIFF tifffile.imwrite("data.tiff", data=np.transpose(cutout))
Documentation for cloud-volume located here
Publications
M. E. Berck, A. Khandelwal, L. Claus, L. Hernandez-Nunez, G. Si, C. J. Tabone, F. Li, J. W. Truman, R. D. Fetter, M. Louis, A. D. Samuel, and A. Cardona. The wiring diagram of a glomerular olfactory system. eLife, 2016.
A. Fushiki, M. F. Zwart, H. Kohsaka, R. D. Fetter, A. Cardona, and A. Nose. A circuit mechanism for the propagation of waves of muscle contraction in Drosophila. eLife, 2016.
T. Jovanic, C. M. Schneider-Mizell, M. Shao, J. B. Masson, G. Denisov, R. D. Fetter, B. D. Mensh, J. W. Truman, A. Cardona, and M. Zlatic. Competitive Disinhibition Mediates Behavioral Choice and Sequences in Drosophila. Cell, 2016.
S. E. Macnamee, K. E. Liu, S. Gerhard, C. T. Tran, R. D. Fetter, A. Cardona, L. P. Tolbert, and L. A. Oland. Astrocytic glutamate transport regulates a Drosophila CNS synapse that lacks astrocyte ensheathment. Journal of Comparative Neurology, 2016.
P. Schlegel, M. J. Texada, A. Miroschnikow, A. Schoofs, S. Hückesfeld, M. Peters, C. M. Schneider-Mizell, H. Lacin, F. Li, R. D. Fetter, J. W. Truman, A. Cardona, and M. J. Pankratz. Synaptic transmission parallels neuromodulation in a central food-intake circuit. eLife, 2016.
M. F. Zwart, S. R. Pulver, J. W. Truman, A. Fushiki, R. D. Fetter, A. Cardona, and M. Landgraf. Selective inhibition mediates the sequential recruitment of motor pools. Neuron, 2016.
A. Kreshuk, J. Funke, A. Cardona, and F. A. Hamprecht. Who is talking to whom: Synaptic partner detection in anisotropic volumes of insect brain. Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics), 2015.
T. Ohyama, C. M. Schneider-Mizell, R. D. Fetter, J. V. Aleman, R. Franconville, M. Rivera-Alba, B. D. Mensh, K. M. Branson, J. H. Simpson, J. W. Truman, A. Cardona, and M. Zlatic. A multilevel multimodal circuit enhances action selection in Drosophila. Nature, 2015.
J. T. Vogelstein, Y. Park, T. Ohyama, R. A. Kerr, J. W. Truman, C. E. Priebe, and M. Zlatic. Discovery of brainwide neural-behavioral maps via multiscale unsupervised structure learning. Science, (6182)344:386–392, 2014.
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